The recombinant CcCK2α and CcCK2β proteins were purified by affinity chromatography to homogeneity, after overexpression in Escherichia coli. CcCK2α catalytic subunit presents the characteristic and conserved features of a typical protein kinase, similar to the regulatory CcCK2β subunit, that also possess the conserved features of regulatory CK2β subunits, as revealed by comparison of their predicted amino acid sequences with other eukaryotic species. capitata by the 5′/3′ RACE and RT-PCR methods, respectively. We present here the construction of the cDNA molecules of the CK2α and CK2β subunits from the medfly C. Protein kinase CK2 is a ubiquitous Ser/Thr kinase that is highly conserved among eukaryotes it is a heterotetramer composed of two catalytic (α) and a dimer of regulatory (β) subunits. The Mediterranean fruit fly Ceratitis capitata is an insect capable of wreaking extensive damage to a wide range of fruit crops.
